After talking with Lindsey, we were able to get a calibration coordinate system to achieve this type of functionality. Users can define a calibration coordinate system and then all VDM functions return pixel coordinates (relative to top/left of image), as well as calibrated coordinates (relative to calibration axis), and there are also functions to convert between pixel coordinates and calibrated coordinates.
It would be a useful feature addition to Vision software to add functionality to report coordinates of an object in a new coordinate system. A main use case would be if you needed your region of interest to be based off of one coordinate system, but once your object was located, you wanted to report the location based on a different coordinate system. Building in this functionality would eliminate the need to configure calculator steps to translate/rotate the coordinates.
Lindsey Nestor Program Manager, Hardware Services National Instruments