June,
If you use the "AO Write One Update.VI", you will output the signal in software time. If you want this, just make the proper connections to the pins of this VI, and that's it. However, if you want to output a signal in hardware time, you must take a look at the "Getting Started with Analog Output.VI" in LV 6i, and can start to build your VI out of it. If you have LV 6.1, take a look at the "Generate Continous Sinewave.VI" and just get rid of the sinewave building section and place the wave you want to output there.
Hope this information is helpful.
L Aguila
Applications Engineer
National Instruments