All I can do is give some general comments since I don't have the instrument. You don't have any loop so in order to run it, when the VI is stopped, you have to set all of your controls first. This includes the file save button. Once the controls are set, running the VI should give you your graphs and a saved file. If you want to run it again with the same settings, you just have to click the run button. If you want different settings, make the changes and then click the run. Do not use the continuous run button on the toolbar. If you have some specific problems, what can help someone figure out the problem is for you to run the VI and then go to the Operate menu and select Make Current Values Default. Save the VI then. This will then save all of your user inputs and outputs along with the VI.