Hello,
If you open the "IMAQ Vision for LabVIEW VI Reference Help" and navigate to "Vision Utilities >> Calibration VIs >> IMAQ Convert Pixel to Real World", you will see from the general description of this VI that your calibration information is attached to the image by "IMAQ Learn Calibration Template.vi", "IMAQ Set Simple Calibration.vi", or "IMAQ Set Calibration Info.vi". If you are using the first one, then the template image should be a grid of dots rather than a grid of lines.
I would recommend opening up one of the three examples of calibrating that can be found at "C:\Program Files\National Instruments\LabVIEW 7.1\examples\Vision\2.Functions\Calibration". I hope you find these examples useful. Take care!
Regards,
Aaron B.
National Instruments