Hello Sean K,
First off I see that you are in the .NET forums. You might get better responce if you post your questions according to your problem. So for this question I would go with either the DAQ or LabVIEW forums.
So I see that you are getting "The Specified resource is reserved" error when using the Daq assistant. However you state that in Measurement and Automation Explorer you are using the traditional NI-DAQ virtual Channel. So if that is the case then the Traditional DAQ driver is reserving the SCXI card therefore the DaqMX driver is not able to access the card. The reason for this is that the DAQ assistant is a part of the new DaqMX driver. So this is going to cause a resource error because the traditional daq driver has control of the device. So if you go into Measurement and Automation right click on the Traditional NI-DAQ (Legacy) Devices and reset the driver. Then find the device under DaqMX devices and reset the device there. At this point I would open a test panel by right clicking on the device under NI-DAQmx devices and see if your thermal couples can take readings. Then go back to your program and use the daq assistant.
Let me know if this helps you and post back if you have any other questions!
Cheers!
Corby_B