You didn't provide much information about how you are acquiring the images, what speed you are trying to acquire at, how they are connected to the computer, etc. Without more information, it is difficult to answer your question.
I assume you are using two IMAQ boards with four inputs each. The most likely problem is that the four cameras on a single board are not synchronized. If you run wires between the cameras so that they use the same VSYNC signal, they will all start sending an image at the same time. This way, when the card switches between cameras, it maintains the timing of the video signal.
Bruce
Bruce Ammons
Ammons Engineering