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Pixels Conversion

I'm using a CCD with resolution of 768 X 494 pixels and a focusing lens of
magnification 25 times.

When i grab an image using the above and taking distance measurements of
particles in the image in terms of pixels,
what is the physical sense of these measurements in pixels??
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You can calibrate your system to have real world coordinate correspond with pixels. There are three type of correction to obtain the real world measurements from your image: simple calibration (so many units equal so many pixels in the x and y direction), there is perspective calibration(if your camera is at an angle), and non-linear lens distortion. These are all described in more detail in the Vision Concepts manual
LV6\Vision\322916a.pdf

There are also shipping examples to help illustrate these methods under labview6/examples/vision/2. functions/calibration/

Hope this helps,
Brad
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