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IMAQ Cast Image not an image

Dear all ,
I found a problem when i migrated  script from Vision assistance to the LabVIEW code. I want to run continuously then I put the While loop over the migrated code. 
In this case, the first ran was ok, but the next time it has an error " IMAQ Cast Image not an image ". I expected that the output result from the first time was fed back as the input of the next running. Anyone has an idea to fix it out.
I attached the script, VI and sample image as the attach file.
Thanks.

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Oop this is an attached file.

Thanks
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