Depth calculation using focal planes

[Lee from Brazil]

I need someone to work with us to determine the distance between peaks and valleys found on images we get from special surfaces using a microscope and moving it to focus the peak and the valley and planes between them. We need help to get a final image, where everything has good focus, using the good parts that are in focus in each image/layer. Please send a kind of resume to lee@triexsis.com.br.

[BruceAmmons]

I did a lot of work on autofocusing when I built an automated metrology system. The focus measurement method I used could be extended and used for an application like this. It would locate the areas with the best focus and combine them from a series of images to create a single image completely in focus. It would also be possible to create a "depth map" image where it shows the depth at which each part of the image was best focused.

Bruce

[amit_shachaf]

To get good image you can use Image Fusion methods. You acquire series of images in constant spacing and use low-level fusion between them.
I don�t know if the tools exist in LabVIEW. I have Image Fusion Toolbox in Matlab.
One of the best results is achieved by wavelet transform. You can process the images off line after acquisition to get the results.
You can estimate the distances between pick and valleys by contrast measurement on the images in the series. (The translation between the images is a known factor from the acquisition).
What I usually do is to rewrite the algorithm in LabVIEW to get faster response. That is after it is working perfectly in Matlab vision tools.

I have attaché 3 images to demonstrate.

Have fun

Amit Shac
haf