Hi Sebastiaan,
Very silly question and you've probably tried it already - are you relying only on the bus power from the firewire port to power the hard drive? It could be that either the firewire cable is too long or that for some reason the Realtime OS doesn't allow you to pull power off the firewire card or that the firewire card is not able to supply enough power for the hard drive. It could be that there is just enough power there to get the light on the drive to work, but not enough to spin the disk up. You could try using the additional USB-power cable that comes with these drives to supply additional power (try from one of the USB ports on your PXI controller and if that doesn't work try to use a USB port from another PC or laptop). You could also use a standard 5V DC power supply with the correct size adapter if you need to.
Something else - have you tried using the hard drive in one of the USB ports in your PXI8186 controller just to see if it is detected?
Kind regards,
Steven Penney