Hello,
First you need to include the Microsoft Windows Common Controls instrument in your CVI project so you can access the necessary functions to control the ImageList and Toolbar controls (Tools >> Create ActiveX Controller). From the programming side, I would suggest searching MSDN, etc for example programs on how to use an ImageList and Toolbar. As a starting point,
I would suggest taking a look at the MSComctlLib_IImageListGetListImages and MSComctlLib_IToolbarSetImageList functions.
Thanks.