Well, if you will be accessing the data ONLY from within LabVIEW then there are some possibilities for prompting an operator to enter a password to open the file. You just match the password with one that is saved inside the file.
If someone can just launch explorer and open the file in notepad then you have to protect even further by using some form of encryption to scramble the file. Then you can force them to use LabVIEW to view your file.
You have to determine how devious your operators may be. Even if you make a file read-only, it can be changed to RW from explorer very easily.
Michael Aivaliotis