There is a "length of curve" function in the math toolbox. Your main challenge will be to convert your IMAQ image into a matrix. Alternatively, there are a few "mouse-odometer" tools out there that will record the actual distance your mouse has moved. You will have to, of course. trim the data to movements within your actual picture window.
Eric P. Nichols
P.O. Box 56235
North Pole, AK 99705