By real-world, I am assuming units like inches. After calibrating your system (dot grid) it should be possible to set coordinates using real-world values. Look for set coordinate system. I use NI Vision so don't know exact syntax for Vision Builder.
Matthew Fitzsimons
Certified LabVIEW Architect
LabVIEW 6.1 ... 2013, LVOOP, GOOP, TestStand, DAQ, and Vison