Hi Danny,
Are you able to "see" the serial ports within MAX (Measurement & Automation EXplorer)?
I also use multiple COM & LPT ports.
I don't think the problem is related to LV6.1 and VISA not recognizing the serial ports.
Do you see the serial ports in the Device Manager (Control Panel > System)?
It is strange that they appear to disconnect after rebooting the PC.
They should all appear in the Hardware Device Manager, if not, re-install the drivers, some of the driver installation require to be installed in multiple instances (ie: two steps per port!). Although it seems like the installation takes forever, you have to go to the end of the driver installation (even if it seems "stuck" in a loop).
If all the ports appear in the Device M
anager, verify that they all appear in MAX. If they don't, scan for them.
Open MAX, expand "Devices & Interfaces", expand "Ports", and look at what is listed. Since my ports always showed up, I have never tried right clicking "Ports" and selecting "crease new statuic serial port".
Hope this helps,
JLV