Use the Mouse Down event and then use the Point to Row Column VI Server method of the tree control to convert the mouse coordinates to tree coordinates. There is also a good utility function inside <vi.lib\tree\Tree Point Converter.llb> that will convert the mouse coordinates to useful information about which row/column/header is clicked.
The Point to row-col.vi (7.1) is working fine with LabVIEW 7.1 but If I run the same vi in LabVIEW 8.2, it is popping up an error. You have any idea why this is happening?