Hi Biff,
Are you by any chance using DAQmx Base in your application? Most of the references that I can find to this type of error are using DAQmx Base on the PDA.
NI-DAQmx Base Error 130413003 Unable to Lock Memory Chunk
http://digital.ni.com/public.nsf/websearch/E8E3AA97EA29D7D686256E9000698B3C?OpenDocument
App failed. Error code 271645003. Unable to lock memory chunk
http://forums.ni.com/ni/board/message?board.id=250&message.id=6922&requireLogin=False
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http://forums.ni.com/ni/board/message?board.id=170&message.id=81066&requireLogin=False
If none of these suggestions seem to work, post a simplified VI that still exhibits the issue and I'll take a look at it for you. Also, if you do find the solution, please post it here for the community!
Thanks & I hope this helps!
Travis H.
LabVIEW R&D
National Instruments