Setting your scan backlog indicator to be 'disabled and greyed' would not cause this error. It is a good idea to enable that indicator to see what is happening there. Besides trying all the things mentioned in the error message (like increasing buffer, decreasing sample rate), you could decrease the wait time in your loop. Even if you tell LabVIEW how often to run the loop, Windows still has the final say. So this is still system dependent and many things can be a factor, like processor speed, memory, other running applications, etc.
-Alan A.