Hi Pritam,
Thank you for posting on the National Instruments forums.
The DAQmx Timing.VI is used when you need to perform an operation that requries sample timing whether it's Analog Input, Analog Output or Digital IO.
Here is a Developer Zone Article that explains how the Sample Timing VI can be used:
Learn 10 Functions in DAQmx and Handle 80% of Your Data Acquisition Applications.
Hopefully this will tell you what you need to know.
Thank You,
Nick F.
Applications Engineer