Jennifer,
You can send a single byte by sending a single character with a VISA Write. For example writing "A" would send 0x41. You can also right click on the string control and select HEX display to manually type 0xFF.
You mentioned that you want to send a "single pulse". You can send a byte like I described above, but serial communication sends one bit at a time (serial) so you will actually be sending 8 pulses (actually there are start and stop bits as well so 8+) for every byte. Just wanted to make sure this was understood.
Shawn B.
National Instruments