Hello,
This is probably because the Port I/O support is not checked. You have to build an Installer instead of just an executable. Go to the last tab (Installer Settings) in the Application Builder. Check "create installer". Now click on Advanced en check Port I/O support. The rest of the settings can be made as you did before for the executable. Now build the installer. Install this installer on the target PC en check if it works.
Best regards,
RikP
Application Engineering
National Instruments
Rik Prins, CLA, CLED
Software Development Engineer