Though the board is capable of letting you use the lines not used by the handshaking on port C, the driver currently does not support this. So if handshaking is in use on either port, you may not use any portion of port C for general purpose I/O.
However, if you wish, you can get around this with some register-level programming. We don't provide support for this, but it has been done. The best starting place for this would be to download the Measurement Hardware Driver Development Kit which should give you everything you need to get started.
Measurement Hardware Driver Development Kit(DDK)This is also mentioned in Knowledge Base
179F64EWRegards,
Russell
Applications Engineer
National Instruments
http://www.ni.com/support