Hey Pradeep,
Thanks for contacting National Instruments about your issue. CAN does have the ability to go off a single wire, but it sacrifices some signal integrity as it references off the V-. In order to implement this CAN protocol you wiill need to configure it in both software and hardware. In Measurement & Automation Explorere, select Tools->NI-CAN and select the option for single wire in the RT Hardware Configuration. Then follow this
KnowledgeBase in order to connect the pins.
Hope this helps,
Can Wong