I've saved 16bit png's before, you just need the right viewer to see the data. Nothing out there, as far as I can tell, can handle 16bit pngs except Matlab. Is there a reason why you are not saving as tiff? From my experience, the acquisition goes a lot faster since there is no compression involved.
You should also keep in mind that image data is saved as signed integers. Depending on your camera, this will be either 8bit or 16bit. If your camera has a native 12bit resolution, you will see image data in the upper 8bit range (data offset by a value of 2^15). Although, for png's I think data starts from 0. Either way, in order to see your image you'll need to normalize your histogram to the appropriate range.
Message Edited by wpang on 05-02-2005 05:59 PM