I am going to assume that you don't have any hardware filtering on your analog input (which would be ideal).
First, you would want to do it on a timed acquisition (buffered). You would apply the butterworth filter VI after reading the data from the buffer through AI Read VI or higher level VIs (such as AI waveform VI). The output from such VIs would serve as data input into the Butterworth Filter VI. You set your frequency information (cutoffs, sampling freq, etc). The filtered data will come out.
Mark