I am using �Serial communication.vi� to communicate with a RTE-140 bath/Circulator (from NESLab). It works well (write and read). Now, I need to write from a file. So, I replaced �Visa Write� function by �Visa Write from File� function in �Serial communication.vi�. I specified a file at filename terminal. Let�s give this new vi a name �Serial communication from file�. Then, I ran this vi. I can �write�(such as set a setpoint). But �read� doesn�t work (such as read setpoint). It gave me a warning (possible reason: the number of bytes transferred is equal to the input count). Also, �write� only works once. If I run it second time, it doesn�t work. Please help.