The easiest way to do it is passing it as a parameter in your shorcut like this:
There are other alternative ways if you decide to do it programatically...
It depends on the exact functionality that you are trying to achieve, but there are many ways to change the default sequence behavior.
This thread discusses the opening and direct execution of default sequences through the TestStand API. This community example shows how to uses the TestStand API in CVI for loading an unloading sequence files.
I would suggest creating a new post (as this thread is almost 8 years old) with any additional questions, as it would be more likely to be answered quickly.