You can access all of these options via the TestStand API. For instance the Tracing Enabled option is actually a property of the TestStand Engine object...Engine.TracingEnabled. The other option you mentioned, Break At First Step is a property of a TestStand Execution object. All of the properties and methods of the TestStand API are referenced in detail in the Help>>ActiveX API of the Sequence Editor. You can access these properties and methods from within a test sequence by using the ActiveX adapter. You can also access them via your test code modules using the TestStand ActiveX VIs/functions in your preferred programming environment.