I would advise making a brand new forum post. As mentioned, a lot has changed in the past 10 years, and posts on an old thread typically don't gain much visibility if the original poster isn't still around.
If you save a .NET reference in a station global with the .NET adapter it does NOT go away when the sequence execution is done. It keeps the execution appdomain around and it will be re-used with future executions. You will however be unable to unload .NET assemblies loaded by the .NET adapter until those references are cleared.
You are correct! The .NET references persist across executions (which now that I step back and think about it makes sense. They are relative to the application manager for station globals). Thanks, dug9000.