Firoz, the DIO lines on the 7334 are static, not latching, so as you probably know a line must be constantly polled when you are trying to detect the change in the state of a TTL signal. This polling is software-timed and therefore influenced by software latency, increases in the PCI bus traffic, etc. Therefore if the duration of a pulse is too short, or if the DIO lines are not polled fast enough, you might miss a signal. Is it possible that this is part of the problem you are seeing?