Candice,
I'm assuming a few things about your code but I want to verify this with you-
1. Since you are specifying a range from 0:4095 I assume your image is 12-bit, is this true?
2. Also, when you use IMAQ Create are you specifying Greyscale I16 as type?
Now I am not sure what you are expecting to see in these two images. From what I read is the only thing you are expecting to change is the size of the dot?
After you save the image and open it again are seeing the expected image or the washed out version you view when you are saving the image?
Regards
--Michelle
National Instruments