Hi David,
The simple VI that I attached reads an image from file and writes it as a TIFF. If you would like to test it, you can open up one of the images in the Vision >> Images folder (for example, candy.png which is U32) and you will see that it should save it as a U32 TIFF file. If the image you are reading in is I16 (i.e. IMAQ Create has type I16 wired in as a constant) then you should get an appropriate image out. If you are successful with this VI, I would recommend migrating it into your application. I hope this helps give you a good start.
Vu