After creating this thread, the customer addressed a service request to NI Technical Support. The response was the following: it is not possible to simulate an RT target. However, VIs can be run from the host if they are moved under the host computer in the LabVIEW project. In the LabVIEW Real-Time Idea Exchange community, you can find some unsupported procedure for creating a virtual machine that runs LabVIEW RT. The customer was able to create a virtual machine with LabVIEW RT (like Desktop PC), but it doesn't simulate the cRIO architecture.