Take a look at the low level snap example. Put the IMAQ Trigger Drive VI between the IMAQ Configure Buffer and IMAQ Start and set the "trigger drive" input to asserted. This will drive the trigger line as soon as the VI is called. Alternately, you could choose another condition such as "acquisition done" to drive the trigger after the frame is acquired. Please note, as soon as you call IMAQ Close, the trigger line will return to the default tri-state.
Regards,
Brent R.
Applications Engineer
National Instruments