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I have the attached file. Just running it doesn't work. But opening the DAQ assistant, clicking run, and then 'OK' and closing the VI does set the line high.
When you run a code your getting any errors? If NO then use multi meter to check the physical voltage in the port 1 line 6 you should get 5v as output when line is high.You can also use MAX test panel to verify.(MAX-->Devices and interfaces-->USB-6501-->Test panels) in that select port 1 line 6 and make it high then check in multi meter weather you are getting voltage or not.