Yes, you can do it! You just have to write to the digital port continously every time you update one of the user boolean controls (dio lines), and you will be writing to the whole set of dio lines.
If you are going to be using LabVIEW, there are some shipping examples that write to a port or to a digital line, especially designed for E Series boards are yours. In LW/CVI, you can also find similar examples that write to the whole port continuously.
Hope this helps.
L Aguila
Applications Engineer
National Instruments