Hi Tim C.,
so you will get your image without using the IMAQ Toolkit, right? Where do you have problems with the "Image edge detection.vi"? Can you already load your own picture into it? If you have the coordinates from your region of interest, use these values and extract this area from the original picture.
Do you ever get edge detection working without IMAQ or OPen CV?
Currently experimenting with trying basic edge detection (again) in the hopes of basically auto-cropping a basic shape image to remove the excess background and then re-save the image with the excess background removed.
Refer to the Edge Detection with 2D Convolution VI in the labview\examples\Signal Processing\Signal Operation directory for an example of using the Convolution VI.