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HPLC Peak detection and integration

Hello Newsgroup!

I´m a beginner regarding Labview, and my (private) project was to write a
software for a HPLC (Chromatography)-System. I´ve managed do read out the
analog/digital converter via the serial port, and the data is correctly
displayed in an XY-Graph (x: time, y: signal strenght).

Now the next step would be the peak detection whcih should display the
retention time of the peak, and the non plus ultra would be also the area of
the peak.

I´ve player around with the peak detection examples from NI, but i wasn´t
able to detect the peaks in a correct way.

Now my question: I´m sure such peak detection and integration programs have
been written before in LabView - it would be very helpful if anyone could
share this code with me
. Any helpful tips are also greatly appreciated!

Best wishes, and a nice weekend!

Christoph Denk
Message 1 of 3
(2,819 Views)
Christoph,

A few years ago, I was asked to make a HPLC program for a customer (because
LabVIEW was cheaper then a HPLC program!). To still be cheaper, I had to
make it in two days!

It sounds exactly like what you want. It also used IO cards to grab an
analog signal.

As I recall, HPLC programs use base lines. I think my program used the
baseline to get the beginnings of the peaks (zero crossing). I think I also
had some custom routines...

One thing I didn't implement is the detection of "merged peaks". A strategy
to detect those is to take the first and second derivative of the signal,
and do some thesholding and zero crossing on those signals.

Anyway, I'll look for the program. I cannot offer it for use, but I guess it
wouldn't harm if you had a look at it.
It might take some time! Also, I have
to send it to you directly (not to the group).

Regards,

Wiebe.


"Christoph Denk" wrote in message
news:%Srkc.583111$Or1.33862@news.chello.at...
> Hello Newsgroup!
>
> I´m a beginner regarding Labview, and my (private) project was to write a
> software for a HPLC (Chromatography)-System. I´ve managed do read out the
> analog/digital converter via the serial port, and the data is correctly
> displayed in an XY-Graph (x: time, y: signal strenght).
>
> Now the next step would be the peak detection whcih should display the
> retention time of the peak, and the non plus ultra would be also the area
of
> the peak.
>
> I´ve player around with the peak detection examples from NI, but i wasn´t
> able to detect the peaks in a correct way.
>
> Now my question: I´m sure such peak detection and integration programs
have
> been written before in LabView - it would be very helpful if anyone could
> share this code with me. Any helpful tips are al
so greatly appreciated!
>
> Best wishes, and a nice weekend!
>
> Christoph Denk
>
>
>
Message 2 of 3
(2,819 Views)

Just wanted to follow up and ask if there is any example programs anyone can share on this topic.

 

Thanks

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Message 3 of 3
(1,212 Views)