Shiva;
If the pulse you want to generate is TTL type, you can use one of the general purpose counters to do that. To accomplish that with the counter, just configure the counter as Triggered Single Pulse Generation, and setup the gate of the counter to be PFI0. There is a really good example in Labview showing how to do that (Search Examples->I/O Interface->Data Acquisition->Counters).
If the pulse is not TTL, you will need to use the Analog output channel instead. If that is the case, you will need to make the AO Update signal to be the PFI0 pin, and configure the Analog Output task to use an external Update clock. There is a LV example on that too (Search Examples->I/O Interfaces->Data Acquisition->Analog Output).
Hope this helps.
Filipe