If by chance you are using an earlier version of the NI-DAQ driver that supports your card, you may not have Measurement and Automation Explorer on your system. If so, you should use the NI-DAQ Configuration Utility to check your card's functionality. I agree with the first suggestion that something has altered the configuration of the card.
You didn't specify your card, OS, or whether you were using a VI in LabVIEW or an EXE and the run-time engine, so it's difficult to give a good diagnosis.
It may be worth your time to run through the online DAQ Troubleshooting pages in the support section of ni.com:
http://www.ni.com/support/daq/
If you do have an older NI-DAQ driver version, it might also be worth your while to update it so that you get better support on any futu
re problems that crop up.
Best Regards,
John Lum
National Instruments