I attempted to reproduce the problem but everything worked fine in my test. I'm using LabVIEW 6.1 and Matlab 6. I created a .bin file in the Matlab Script Node in LabVIEW and then read the data in Matlab. Everything worked fine. It may be possible the problem is specific to certain versions of either LabVIEW or Matlab. Which version of LabVIEW are you using? Also, do you have any sample code that illustrates the problem?