Unfortunately I don't have a USB-5133 so I can't test this with a physical device, but I haven't been able to reproduce the issue with a simulated device. I know that doesn't really help the situation since you aren't encountering the issue with simulated devices either.
However, I do note that in your screenshots somehow you have channels 0 and channel 1 swapped from what is normally seen -- i.e., channel 0 is in the right dropdown and channel 1 is in the left dropdown. Normally such a configuration isn't possible unless a device has more than 2 channels, since by default channel 0 is in the left dropdown and you can never have the same channel selected in both dropdowns simultaneously, thereby eliminating the possibility of swapping them. Of course from your image we see that this has occurred, so... yeah...
It is possible you have a corrupted configuration file for the device saved to the hard drive. If I recall correctly, upon upgrading (or downgrading) NI-SCOPE versions,the config files are not automatically deleted from the hard drive so that could potentially explain why we see this behavior even in 14.1 which had numerous fixes to the horizontal zoom capabilities. At this point I really only have a couple of suggestions.
If deleting the configuration file doesn't fix the issue, it would be nice if someone else could test this on real hardware.
Looks like deleting the .h5 file fixxed the issue! I have included it for future reference. Thank you very much for your help in resolving this issue!!!
might have to erase the ".doc" on file name. Wouldnt let me send as original file.