As Sal stated, you cannot perform a hardware reference trigger and then proceed to acquire continuously. Additionally, you cannot perform a hardware reference trigger with multiple channels in the scan list. You will have to perform some software manipulation in order to achieve your desired goal. However, there is an NI-DAQmx example that ships with LabVIEW 7.0 or higher that looks like it may work for you. The example is called Cont Acq&Graph Voltage-Analog SW Trigger.vi. In the LabVIEW Example Finder, navigate to Hardware Input and Output >> DAQmx >> Analog Measurements. In here you will find this example. If you take a look at the subVI called Analog SW Trigger.vi, you can see that this is performing some software analysis looking for a trigger point, similar to what Sal mentioned. I haven't used this example much, but if you delete the wire branch from the Analog SW Trigger.vi subVI to the Compound Arithmetic OR, then the VI should run continuously, even if you specify a number of pretrigger scans > 0. I hope this helps.