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Can we write help messages in LABView VIs? Like there will be a tab written manual on it. Whenever it will be clicked, a message will pop up showing the given instruction.
You can do lots of things. It all comes down to how much effort you are willing to put into it. You can go to the Documentation page on a control's properties and enter in a tip strip which will appear to the user when they hover over a control. You can also create a whole help manual that you can open via a menu item or button. If you do a search for writing help manuals you will see that this has come up. The easiest thing to do is to create an HTML-based manual. You can also create your own popus, if you wish. The Example FInder (Help -> Find Examples) has some popups.
