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Good day to you. You can start off by looking at the example VI. There is an example for pattern matching called Pattern Matching Example.vi. Use the example finder to look for it. In the example you can see that the property node is used to set the ROI of the image. Probably you can modify this VI to make it simpler for your application.
two common ways or analyzing images on smaller ROI subsets is to do an extract image (extracks smaller portion of image into new image space) or to creat a mask and perform your analysis within a masked region.