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My issue is i have a 3d scene with a couple of objects like light sources, when there is change in the brightness in a 3D scene how to measure it and get the values for region of interest, please suggest some methods.
The pixel brightness would of course depend on the location of the camera. You can export the current view as a picture ("Render to Image" method of the 3D Picture Control) will give you an array of pixel values.