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ImageJ thresholding problem!

I am facing a problem with thresholding while I am using Imagej. I am analyzing 2160 images as one stack. I usually divide them as 3 groups (720 images / each group) but this time I got three different values for threshloding (converting images to Black and white). Is any one has any idea why I got this?Imagej

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Are you aware of the fact that this is not the ImageJ forum? You can get help here for LabVIEW. Maybe you should try to seek help in the ImageJ forum...

http://forum.imagej.net/

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