The manual doesn't state it, but most serial devices require a termination character at the end of each command. Hyperterminal does this by default. Try appending a CR or LF character (or both) and see if that works. You can do this several ways. If you're using a string control for input, turn on \ Codes Display and put \n (LF) or \r (CR) at the end of your string. You can use Concantanate Strings with the constants on the string palette. You can use the VISA Property node (see VISA Configure Serial Port) and add the property Serial Settings:Serial End Mode for Writes, enable it, and set the termination character to the correct hex value (0A = LF, 0D = CR).