Dan,
What is the baud rate of your serial card set at in Measurement and Automation Explorer (MAX)? Make sure that this matches whatever you are trying to set in your VB application.
For your reference, here is a
KB about what baud rates are supported by NI serial boards.
What NI serial board do you have?
This is a duplicate of this
post, try to post only one time if it's the same issue!
JenK