Hi chrisjohnson,
yes, when you have the card configured to use the single-wire physical layer, you can set the 'Transceiver Mode' to 'Single Wire Wakeup' to drive a higher voltage to the bus to wake up other sleeping nodes.
If you use the single-wire physical layer, and the transceiver on teh PCI-CAN card is set to sleep ('Transceiver Mode' set to 'Sleep'), the transceiver will wake up as soon as a high-voltage is detected on the CAN line.
-B2k